Snake Meat......source of chinese virus

[badger2]

First seen at Avian Flu Talk, this thread reported on Tencent yesterday. At that forum, they are currently tapping bitchute. The reader can decide on this disturbing video:

China Footage, 10 Days In
https://www.bitchute.com/yuAG7tjHDJ8I

 

[badger2]

Uwantsun

 

[badger2]

Current stats are at post #279.

 

[badger2]

They have grave concerns about what the supply chain reduction is going to do to us and others. Some downplayed the whole thing as not an emergency yet.

Coronavirus: House Foreign Affairs Committee (see especially @ timepoint 59:42 China's Influence)

 

[badger2]

The headlines say 7 Feb 2020 Flu Has Killed 10,000 Americans As the World Worries Over Coronavirus.
Yes, and we're going to continue to worry, especially since there's a flu vaccine and 2019-nCoV is now mutating against non-vaccine therapies.

 

[badger2]

Currently, 635 deaths (post #279). HIstorically, SARS total was 800.

7 Feb 2020 400 Million Quarantined
https://www.zerohedge.com/geopoliti...on-people-lockdown-guangzhou-joins-quarantine

 

[badger2]

zerohedge.com/geopolitical/more-than-400-million-people-lockdown-guangzhou-joins-quarantine

 

[badger2]

Helen Branswell
mobile.twitter.com/HelenBranswell/status/1225802856478822400
'Based on 17,000 cases, 82% are mild, 15% severe, 3% critical....was not broken down by age.'

 

[badger2]

State-of-the-art test results should not take long.

AFT, 4 minutes ago: Cruise Ship to Dock in Bayonne
https://www.nypost.com/2020/02/06/c...onne-will-test-12-passengers-for-coronavirus/

 

[badger2]

Avian Flu Talk: 'Yes, the U.S. is counting on holding through the first wave until summer. Remember, few experts believe these are actual numbers. They are Soviet-style numbers meant for general consumption and pacifying the public....Chinese citizen journalists have disappeared....they have cracked down on all social media posts.'

 

[badger2]

41 more cases on the Japanese cruise ship (6 hrs. ago) and the Chinese whistleblower warned in early December before being silenced (@ timepoint 0:16):



AFT forum: ' Why no updates about U.S. patients?....I think some bigger news may be coming today. Govt. and msm seem to time bad news for late afternoon Fridays.'

 

[badger2]

The youtube video is 7 Feb 2020, Chinese Doctor Who Warned of Coronavirus Dies (1,432 comments)

 

[badger2]

Bioweapons theories aside, the Italians' experience with plague ships, etc., have zeroed-in on important mutations showing that 2019-nCoV has mutated one amino acid from the closest bat-like SARS to it. ORF8-S isoform is from Rhinolophus sp.:

Genomic Variance of the 2019-nCoV Coronavirus, University of Bologna, Italy
'....Despite low heterogeneity of 2019-nCoV genomes, we could identify at least two hyper-variable genomic hotspots, one of which is responsible for a sering/leucine variation in the viral ORF8 (open reading frame)-encoded protein. Finally, we perform a full proteomic comparison with other coronaviridae, identifying key aminoacidic differences to be considered for antiviral strategies deriving from previous anti-coronavirus approaches.....We could report also the specific amino acids that changed between SARS and nCoV, with potential implication in epitope definition and possible repurposing of anti-SARS drugs and vaccines.
....
Our analysis found low variability (>99% sequence identity) within the 56 2019-nCoV genomes available at the time of writing, with only two core positions of high variability, one a silent variant in the ORF1b locus, and the other as an aminoacid polymorphism in ORF8. The mutation in ORF8 resulting in one of its two variants, ORF8-L and ORF8-S, is predicted to be affecting the structural disorder of the protein. Specifically, the amino acidic region aa83-aa89 is more likely to be disordered in the ORF-8-S isoform....specific amino acid divergences compared to BCoVs and SARS. These findings shed a cautiously optimistic light on the possibility of finding effective treatment for this novel coronavirus, starting from already existing anti-beta-coronaviridae compounds (Anand K, Coronavirus Main Proteinase (3CLpro) Structure: Basis for Design of Anti-SARS Drugs, Science [2003] V. 300:1763-67), which will be dealing with a relatively homogenous viral population.....relationships between bat and human coronaviridae have been discovered for SARS [3]and more recently also for 209-nCoV [4], suggesting events of inter-species transmissions [5]'
(Ceraolo C, Giorgi FM, Genomic Variance of the 2019-nCoV Coronavirus)

Reference [4] is Benvenuto et al, previously posted, which mentions the report of Ji et al (also previously posted) for Bungarus multicinctus. Thus, the leucine change in ORF8 may reflect adaption to cold-blooded hosts.

 

[badger2]

Comparing ACE2-leucine, there are 34 refs at Pubmed:

ACE2 / Leucine
ace2 leucine - PubMed - NCBI

 

[badger2]

This from the UW campus:

7 Feb 2020
Coronavirus vaccine in the works, UW-Madison experts say | WisBusiness
'....less than two months.'

 

[badger2]

Avian Flu Talk is reporting pangolin as an intermediate host, while here we are following the ACE2-leucine trajectory for snake (post #314). One study links DPP4 (posted earlier in the thread linking Thai oseltamivir), to leucine:

Mar 2017 DPP4 / Leucine Aminopeptidase
Serum protease activity in chronic kidney disease patients: The GANI_MED renal cohort. - PubMed - NCBI

We note that the second hotspot for 2019-nCoV was position 494 (post #299), but also the leucine at position 455.

Another important link in the studies of post #314 is the mouse hepatitis virus strain A59 (previous posts) link:

WHV A59 / Proteosomal Inhibitor MG132
Severe acute respiratory syndrome coronavirus replication is severely impaired by MG132 due to proteasome-independent inhibition of M-calpain. - PubMed - NCBI
'....strongly inhibits SARS-CoV viral life cycle....bortezomib actually led to increased viral titers and pathology....Since MG132, a tripeptide aldehyde (benzyloxycarbonyl-leucyl-leucyl-leucinyl, or ZLLLa1, comprised an unparalleled capability of inhibiting SARS-CoV replication
, we hypothesized that inhibition of another proteolytic enzyme is responsible for inhibition of viral titer: m-calpain, a cysteine protease.'

When immediately searching for calpain links to snakes, we come up with both suspects for intermediate hosts (Bungarus and Naja) of 2019-nCoV. We'll link Naja first, because of the plant potyvirus link to papain (previous posts):

Snake / Calpain
snake calpain - PubMed - NCBI

Naja, Taiwan Cobra Inhibits Cysteine Protease
Purification and characterization of a new cystatin inhibitor from Taiwan cobra (Naja naja atra) venom. - PubMed - NCBI
'....S-carboxymethylpapain....inhibits cysteine proteases of the papain family....most closely related to the recently described human cystatin M which also has a similar five-residue insertion starting at position 76.'

This is an interesting clue to why mice and rats do not figure into the 2019-nCoV host hypothesis (previous posts):

Bungarus multicinctus / Apoptosis of Rat Neurons
Beta-bungarotoxin is a potent inducer of apoptosis in cultured rat neurons by receptor-mediated internalization. - PubMed - NCBI

We next link another Thai med used against 2019-nCoV, ritonavir, to calpain:

(2002) HIV Protease / m-Calpain
Ritonavir inhibition of calcium-activated neutral proteases. - PubMed - NCBI
'....Both human HIV protease and calpains share a similar secondary structure, where the active site is flanked by hydrophobic regions. Ritonavir, a hydrophobic HIV protease inhibitor, also inhibits calpain activity.'

 

[badger2]

Bad News, Late Friday Afternoon

720 deaths (post #279), quicky ready to overtake historical SARS stats (800 total cases).

 

[Frankeneinstein]

Seems strange, it might be in both warm blooded and cold blooded animals.

good point, but if a snake eats a rat and a human eats the snake then it may have some kind of crossover affect that results in this type of virus in humans

 

[badger2]

#318: Precisely: the virus may not have had time to mutate, as was suggested in a previously-posted study. But then, from what host did the leucine mutation come from that used to be a serine in the most closely-related bat virus? Could cold-shock by being outside the bat host mutate ORF8 (in trans [italics])? Or had it already mutated in a certain individual bat? That is to say,did it mutate on some surface, like pangolin or snake scales?

Note that in post #316, we did not post the full sentence, which reads: ' Cobra cystatin strongly inhibits cysteine proteases of the papain family, but not calpain.' This suggests that Thai use of anti-HIV drugs indeed does link to calpains, as was shown in the pubmed study (HIV Protease / m-Calpain), though the controversy arises in opposing studies such as this:

Ritonavir Does Not Inhibit Calpain
Ritonavir does not inhibit calpain in vitro. - PubMed - NCBI

 

[Circe]

Currently, 635 deaths (post #279). HIstorically, SARS total was 800.

7 Feb 2020 400 Million Quarantined
https://www.zerohedge.com/geopoliti...on-people-lockdown-guangzhou-joins-quarantine

Yeah, when the death total hits 800 (the approx. SARS death total in 2003) there will be some alarm expressed, I think. That will be this Sunday, I project.